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primescripttm rt reagent kit  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm rt reagent kit
    Primescripttm Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 14654 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gdna+eraser/PrimeScript+RT+Reagent+Kit+with+gDNA+Eraser/pmc13091180-119-13-18
    Average 99 stars, based on 14654 article reviews
    primescripttm rt reagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Comparative effects of bile salts and free bile acids on intestinal health of grouper ( Epinephelus spp.) fed a high cottonseed protein concentrate diet
    Article Snippet: In accordance with the methods outlined in previous studies ( ; ), total RNA was extracted from intestinal and hepatic tissues using RNAiso Plus (catalog no. 9109, TaKaRa Biotechnology [Dalian] Co., Ltd., Dalian, Liaoning, China). .. After quantifying RNA concentration with a NanoDrop One/OneC Microvolume UV–Vis Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), 1 μg of RNA was reverse-transcribed into cDNA using the PrimeScript RT reagent Kit with gDNA Eraser (catalog no. RR047A, TaKaRa Biotechnology [Dalian] Co., Ltd., Dalian, Liaoning, China). .. The mRNA expression levels were subsequently quantified by RT-qPCR with TB Green Premix Ex Taq II (catalog no. RR820A, TaKaRa Biotechnology [Dalian] Co., Ltd., Dalian, Liaoning, China) on a QuantStudio 5 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).

    Spectrophotometry:

    Article Title: Comparative effects of bile salts and free bile acids on intestinal health of grouper ( Epinephelus spp.) fed a high cottonseed protein concentrate diet
    Article Snippet: In accordance with the methods outlined in previous studies ( ; ), total RNA was extracted from intestinal and hepatic tissues using RNAiso Plus (catalog no. 9109, TaKaRa Biotechnology [Dalian] Co., Ltd., Dalian, Liaoning, China). .. After quantifying RNA concentration with a NanoDrop One/OneC Microvolume UV–Vis Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), 1 μg of RNA was reverse-transcribed into cDNA using the PrimeScript RT reagent Kit with gDNA Eraser (catalog no. RR047A, TaKaRa Biotechnology [Dalian] Co., Ltd., Dalian, Liaoning, China). .. The mRNA expression levels were subsequently quantified by RT-qPCR with TB Green Premix Ex Taq II (catalog no. RR820A, TaKaRa Biotechnology [Dalian] Co., Ltd., Dalian, Liaoning, China) on a QuantStudio 5 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).

    Reverse Transcription:

    Article Title: Comparative effects of bile salts and free bile acids on intestinal health of grouper ( Epinephelus spp.) fed a high cottonseed protein concentrate diet
    Article Snippet: In accordance with the methods outlined in previous studies ( ; ), total RNA was extracted from intestinal and hepatic tissues using RNAiso Plus (catalog no. 9109, TaKaRa Biotechnology [Dalian] Co., Ltd., Dalian, Liaoning, China). .. After quantifying RNA concentration with a NanoDrop One/OneC Microvolume UV–Vis Spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA), 1 μg of RNA was reverse-transcribed into cDNA using the PrimeScript RT reagent Kit with gDNA Eraser (catalog no. RR047A, TaKaRa Biotechnology [Dalian] Co., Ltd., Dalian, Liaoning, China). .. The mRNA expression levels were subsequently quantified by RT-qPCR with TB Green Premix Ex Taq II (catalog no. RR820A, TaKaRa Biotechnology [Dalian] Co., Ltd., Dalian, Liaoning, China) on a QuantStudio 5 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA).

    Article Title: BUD23 is associated with malignancy and correlates with immune infiltration in NSCLC
    Article Snippet: RNA was extracted from the cells using TRIzol ® (Invitrogen; Thermo Fisher Scientific, Inc.). .. RT was performed using the PrimeScript TM RT Reagent Kit with gDNA Eraser (Perfect Real Time; cat. no. RR047A; TaKaRa Bio Inc.) containing reverse transcriptase, RT buffer, dNTP mix, oligo(dT) primers, random primers and gDNA Eraser. ..

    Article Title: 3D-printing flexible PLGA scaffold modified with bioorthogonal IGF-1 for skin regeneration
    Article Snippet: On day 7, total RNA was extracted using TRIzol Reagent (Invitrogen). .. And the reverse transcription was governed using PrimeScript RT Reagent Kit with gDNA Eraser (Perfect Real Time, TaKaRa). ..

    cDNA Synthesis:

    Article Title: Heterologous production of epothilones in Streptomyces albus J1074 via promoter engineering of the biosynthetic gene cluster and precursor supply enhancement
    Article Snippet: Total RNA was extracted using the RNAprep Pure Kit (Tiangen Biotech (Beijing) Co., Ltd., cat. no. DP430). .. Genomic DNA (gDNA) removal and complementary DNA (cDNA) synthesis were performed using the PrimeScript RT Reagent Kit with gDNA Eraser (Takara Bio Inc., Shiga, Japan; cat. no. RR047A). .. Real-time PCR was carried out on a StepOnePlus Real-Time PCR System (Applied Biosystems, Waltham, MA, USA) using TB Green Premix Ex Taq (Takara Bio Inc., cat. no. RR420A), following the manufacturer's protocol.

    Article Title: Multi-omics analysis reveals RBPJ-mediated regulation of EGF/ACTN2 / MYPN / COL21A1 in fibroblast during oviduct functional remodeling of duck
    Article Snippet: .. Total RNA was extracted from fibroblasts (OE and C) and oviduct tissue (laying and ceasing laying) using TRIzol® Reagent (15596026, Invitrogen, USA) according to the manufacturer’s instructions. cDNA synthesis was performed using the PrimeScript TM FAST RT reagent Kit with gDNA Eraser (RR092A, Takara, China). .. Gene-specific primers for qPCR were designed using the Primer-BLAST tool (National Center for Biotechnology Information, NCBI), with Beta-actin ( ACTB ) as the internal reference gene to normalize target gene expression.

    Synthesized:

    Article Title: Dynamic changes in genes and metabolites during embryonic development of Jiangshan black-bone chickens
    Article Snippet: .. cDNA was synthesized from total RNA using the PrimeScriptTM FAST RT Reagent Kit with gDNA Eraser (RR092A, Takara, China). .. Gene-specific primers, listed in , were designed via the NCBI Primer-BLAST tool, with GAPDH serving as the internal housekeeping gene for normalization. qRT-PCR was conducted on a QuantStudioTM 3 Real-Time PCR System (Applied Biosystems, USA) utilizing Universal SYBR® Green qPCR Master Mix (HY-K0501A, MCE, USA).

    Article Title: Melatonin-incorporated brain extracellular matrix hydrogel enhances NSCs mitochondrial metabolism to promote neuroregeneration via the AMPK-PGC-1α-NRF1/TFAM axis after spinal cord injury
    Article Snippet: Total RNA was extracted from cells using the Eastep Super Total RNA Extraction Kit (LS1040, Shanghai Promega, China) according to the manufacturer's protocol. .. First-strand cDNA was then synthesized from the extracted RNA using the PrimeScript Fast RT Reagent Kit with gDNA Eraser (RR092A, Takara, Japan). .. Quantitative PCR was subsequently performed using TB Green Premix Ex Taq II Fast (CN830A, Takara, Japan) on a QuantStudio 5 real-time PCR system (Applied Biosystems, USA).

    Article Title: Third-trimester estradiol is associated with isolated maternal hypothyroxinemia and attenuated TSH-FT4 coupling: evidence from rats and human astrocytes.
    Article Snippet: .. RT-qPCR: Total RNA was extracted using TRIzol Reagent (Invitrogen; Cat# 15596018CN), and cDNA was synthesized using the PrimeScript RT reagent Kit with gDNA Eraser (Takara Bio; Cat# RR047A). .. Amplification was performed using TB Green Premix Ex Taq II (Tli RNaseH Plus) (Takara Bio; Cat# RR820A) on a StepOne Plus Real-Time PCR System (Applied Biosystems).



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